Question 1 of 20
The graph shows the change in a moth population over time. Dark moths increased after industrial pollution darkened tree bark. This is an example of:
ASpontaneous generation
BArtificial selection
CGenetic engineering
DNatural selection
Explanation
📌 This is the classic peppered moth example of natural selection.
Before pollution: light bark → light moths camouflaged → survived
After pollution: dark bark → dark moths camouflaged → survived
The environment selected for the trait that provided camouflage.
Before pollution: light bark → light moths camouflaged → survived
After pollution: dark bark → dark moths camouflaged → survived
The environment selected for the trait that provided camouflage.
Question 2 of 20
Lipids store roughly twice as much energy per gram as carbohydrates. Which property of lipid molecules best explains this?
AThey are insoluble in water, so they take up less space when stored
BLong hydrocarbon chains contain many C–H bonds that can be oxidized to release energy
CThey form longer polymer chains than carbohydrates
DThey contain more nitrogen atoms than carbohydrates
Explanation
Lipid fatty-acid tails are long chains of carbon–hydrogen bonds. Oxidizing each C–H bond releases energy, so the more C–H bonds per gram, the more energy is stored. Carbohydrates have fewer C–H bonds per carbon because oxygen atoms are already attached. Distractors: water insolubility relates to *where* lipids are stored, not how much energy they hold. Lipids aren't true polymers. Lipids contain almost no nitrogen — that's a feature of proteins and nucleic acids.
Question 3 of 20
The carbon cycle involves:
Aonly the atmosphere
Bonly living organisms
Cthe movement of carbon through living and non-living systems
Donly the ocean
Explanation
📌 Carbon moves through: atmosphere (CO₂) → photosynthesis → organisms → respiration/decomposition → atmosphere.
Also: fossil fuels → combustion → atmosphere.
Also: fossil fuels → combustion → atmosphere.
Question 4 of 20
In the food web below, what would happen if all the frogs were removed? 
ANothing changes
BAll organisms die
CInsect population increases and snake population decreases
DSnake population increases
Explanation
📌 If frogs are removed:
• Insects (grasshoppers) they ate would INCREASE (no predator)
• Snakes that ate frogs would DECREASE (less food)
• This demonstrates how removing one species affects the entire food web.
• Insects (grasshoppers) they ate would INCREASE (no predator)
• Snakes that ate frogs would DECREASE (less food)
• This demonstrates how removing one species affects the entire food web.
Question 5 of 20
Which graph correctly shows exponential growth?
ANeither
BBoth
CA
DB
Explanation
📌 Exponential growth = J-shaped curve (slow start, rapid increase)
Linear growth = straight line (constant rate)
Graph A shows exponential growth.
Linear growth = straight line (constant rate)
Graph A shows exponential growth.
Question 6 of 20
All three domains of life (Bacteria, Archaea, and Eukarya) share which structural feature?
AA membrane-bound nucleus
BRibosomes for protein synthesis
CMulticellular organization
DChloroplasts for photosynthesis
Explanation
All cellular life — Bacteria, Archaea, and Eukarya — uses ribosomes to build proteins. Distractors: a membrane-bound nucleus is found only in Eukarya (Bacteria and Archaea are prokaryotic). Multicellularity appears only in some Eukarya — most life is unicellular. Chloroplasts are organelles found only in plants and algae (a subset of Eukarya).
Question 7 of 20
Which molecule carries energy from glucose breakdown to the electron transport chain?
AH2O
BNADH
CATP
DCO2
Explanation
📌 NADH and FADH2 are electron carriers. They transport high-energy electrons from glycolysis and Krebs cycle to the ETC.
Question 8 of 20
Which pair of processes drives the most direct exchange of CO₂ between living organisms and the atmosphere?
APhotosynthesis (releases CO₂) and cellular respiration (absorbs CO₂)
BTranspiration (releases CO₂) and condensation (absorbs CO₂)
CPhotosynthesis (absorbs CO₂) and cellular respiration (releases CO₂)
DNitrogen fixation (absorbs CO₂) and decomposition (releases O₂)
Explanation
Photosynthesis consumes atmospheric CO₂ to build glucose (CO₂ → sugar), while cellular respiration breaks glucose back down to CO₂ and water. Together they form the short-term carbon cycle between the biosphere and atmosphere. Distractor B reverses the direction. Transpiration moves water vapor, not CO₂. Nitrogen fixation moves nitrogen, not carbon.
Question 9 of 20
A student designed an experiment to test how light affects plant growth. Identify the components of this experiment. 
AIndependent: light; Dependent: plant height
BIndependent: plant height; Dependent: light
CIndependent: water; Dependent: soil
DIndependent: temperature; Dependent: light
Explanation
📌 Independent variable = what you change (light)
Dependent variable = what you measure (plant height)
Control group = no light (to compare against)
Constants = same soil, water, temperature, pot size
Dependent variable = what you measure (plant height)
Control group = no light (to compare against)
Constants = same soil, water, temperature, pot size
Question 10 of 20
A theory in science is:
Aa well-supported explanation based on extensive evidence
Ba proven fact that can never change
Can untested guess
Dthe same as a hypothesis
Explanation
📌 A scientific theory is well-tested, widely accepted, and explains a broad range of observations.
It is NOT just a guess — that's a hypothesis.
It is NOT just a guess — that's a hypothesis.
Question 11 of 20
In a DNA nucleotide, which component carries the genetic code through its specific sequence?
ADeoxyribose sugar
BHydrogen bond
CNitrogenous base
DPhosphate group
Explanation
The nitrogenous base (adenine, thymine, guanine, or cytosine) is the part of a DNA nucleotide that varies — its sequence along the strand IS the genetic code. The phosphate and deoxyribose sugar are identical in every nucleotide and form the structural 'backbone' of the DNA strand; they don't store information. Hydrogen bonds hold the two strands together at the bases but are not part of a single nucleotide.
Question 12 of 20
Epigenetics studies how:
Agene expression changes without altering DNA sequence
Bcells divide
Corganisms evolve
DDNA sequence changes
Explanation
📌 Epigenetics = changes in gene expression WITHOUT changing DNA sequence.
Mechanisms: DNA methylation, histone modification.
Affected by environment, diet, stress.
Mechanisms: DNA methylation, histone modification.
Affected by environment, diet, stress.
Question 13 of 20
Which organelle is labeled X in the cell diagram below? 
ANucleus
BMitochondria
CGolgi apparatus
DEndoplasmic reticulum
Explanation
📌 Organelle X has the bean-shaped structure with inner folds (cristae) → Mitochondria
Mitochondria = powerhouse of the cell = produces ATP through cellular respiration
C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + ATP
Mitochondria = powerhouse of the cell = produces ATP through cellular respiration
C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + ATP
Question 14 of 20
Which class of biomolecule is built from nucleotide monomers?
ANucleic acids (DNA and RNA)
BLipids
CCarbohydrates
DProteins
Explanation
Nucleic acids (DNA, RNA) are polymers of nucleotide monomers — each nucleotide is a phosphate + sugar + nitrogenous base. Distractors: Proteins are polymers of *amino acids*. Carbohydrates are polymers of *monosaccharides* (e.g., glucose). Lipids are not true polymers — they assemble from fatty acids and glycerol but don't repeat a single monomer.
Question 15 of 20
In a Punnett square cross Bb × Bb, what fraction of offspring are expected to be homozygous recessive (bb)?
A25%
B50%
C0%
D75%
Explanation
📌 BB:Bb:bb = 1:2:1
bb = 1/4 = 25%
bb = 1/4 = 25%
Question 16 of 20
Use the Punnett square to determine the probability of offspring with the homozygous recessive (aa) genotype.
A0
B3/4 (75%)
C1/4 (25%)
D1/2 (50%)
Explanation
Aa × Aa cross: AA, Aa, Aa, aa. Only one box (aa) shows the homozygous recessive: 1/4 = 25%.
Question 17 of 20
The Hardy-Weinberg principle states that allele frequencies remain constant when:
Athe population is very small
Bthere is no evolution occurring
Cnatural selection is strong
Dmutations are common
Explanation
📌 Conditions: no mutation, no migration, random mating, large population, no natural selection.
Any violation → evolution occurs.
Any violation → evolution occurs.
Question 18 of 20
During which phase of the cell cycle does DNA replication occur? 
AG1 phase
BM phase
CS phase
DG2 phase
Explanation
📌 DNA replication occurs during S phase (Synthesis) of interphase.
Interphase: G1 → S → G2, then Mitosis.
Interphase: G1 → S → G2, then Mitosis.
Question 19 of 20
Alternative splicing allows:
Amutations to be prevented
BDNA to be repaired
Cchromosomes to separate
Done gene to code for multiple proteins
Explanation
📌 One gene → multiple proteins by including/excluding different exons. Increases protein diversity without needing more genes.
Question 20 of 20
Why does the left ventricle have a thicker muscular wall than the right ventricle?
AIt contains valves that the right ventricle lacks
BIt must pump blood at higher pressure to reach the entire body
CIt is in direct contact with the lungs
DIt receives more deoxygenated blood from the body
Explanation
The left ventricle pumps oxygenated blood through the *systemic circuit* — every tissue in the body — which requires high pressure. The right ventricle only needs to push blood a short distance to the lungs (pulmonary circuit), so its wall is thinner. Distractors: the left ventricle pumps *oxygenated* blood, not deoxygenated. Both ventricles have valves (AV and semilunar). The right ventricle, not the left, is closer to the lungs.